Multiplex PCR for Differentiation ofMycoplasma mycoidesCluster from other Mycoplasma
نویسندگان
چکیده
منابع مشابه
Further evaluation of a multiplex PCR for differentiation of Salmonella paratyphi A from other salmonellae.
Salmonella enterica serovar Paratyphi A is a causative agent of paratyphoid fever. The clinical syndrome caused by paratyphoid fever overlaps with other febrile illnesses and cannot be distinguished from typhoid fever. Conventional methods used for diagnosis are time consuming, costly, and labor-intensive. We evaluated the specificity, sensitivity, and application of a multiplex polymerase chai...
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Methods The PCR-based detection technique for five Salmonella species was tested under O.I.E. requirements, to determine specificity, sensitivity, and repeatability. Primers were calculated by OLIGO software and produced with HPLC purification. Amplification was conducted by a T3000 Biometra PCR-machine. Testing was performed on a panel of five basic agents: Salmonella enterica ser. Enteritidis...
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Two new primer sets of a 766- and a 344-bp fragment were introduced into the conventional Bruce-ladder PCR assay. This novel multiplex PCR assay rapidly and concisely discriminates Brucella canis and Brucella microti from Brucella suis strains and also may differentiate all of the 10 Brucella species.
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Mettifogo E., Buzinhani M., Buim M.R., Timenetsky J. & Ferreira A.J.P. 2015. Evaluation of a PCR multiplex for detection and differentiation of Mycoplasma synoviae, M. gallisepticum, and M. gallisepticum strain F-vaccine. Pesquisa Veterinária Brasileira 35(1):13-18. Departamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de ...
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We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169 C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.
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ژورنال
عنوان ژورنال: Journal of Applied Animal Research
سال: 2003
ISSN: 0971-2119,0974-1844
DOI: 10.1080/09712119.2003.9706440